ISO 10272-2-2017 amd1-2023 PDF

Full title and description

ISO 10272-2:2017/Amd 1:2023 — Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 2: Colony-count technique — Amendment 1: inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and changes in the performance testing of culture media. This document is an amendment to ISO 10272-2:2017 and supplements the colony-count technique with additional confirmation/identification options and updated media performance criteria.

Abstract

Amendment 1 (2023) to ISO 10272-2:2017 updates the horizontal colony-count method for Campylobacter spp. by adding methods for molecular confirmation and identification of thermotolerant Campylobacter spp., and by revising the procedures and performance testing requirements for culture media used in enumeration. The amendment clarifies confirmation workflows and performance verification to support reliable colony counting in food, feed and related environmental samples.

General information

• Status: Published (International Standard — amendment published).
• Publication date: 2023 (Amendment published January 2023; effective publication listed as 25 January 2023 in some records, with national adoptions showing early Feb 2023).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 07.100.30 — Food microbiology.
• Edition / version: ISO 10272-2:2017 with Amendment 1 (ISO 10272-2:2017/Amd 1:2023), Edition 1 of the amendment (2023).
• Number of pages: 24 (amendment document length).

Scope

This amendment applies to ISO 10272-2:2017 (the colony-count technique) and extends the existing colony-count protocol by: (a) providing validated approaches for molecular confirmation and identification of thermotolerant Campylobacter spp. as part of the confirmation workflow; and (b) updating the performance testing/verification requirements for selective and non-selective culture media used in enumeration. The method remains applicable to food products intended for human consumption, animal feed, environmental samples in food/feed production and handling, and primary production samples (e.g., animal faeces, dust, swabs).

Key topics and requirements

• Inclusion of molecular confirmation and identification procedures for thermotolerant Campylobacter spp. (e.g., validated PCR-based confirmation or sequencing approaches) to be used alongside phenotypic confirmation steps.
• Revised performance testing and verification criteria for culture media used in colony-count procedures (media quality control, performance panels, acceptance criteria).
• Clarified colony-count technique workflow (sample preparation, plating, incubation conditions and enumeration principles consistent with ISO 10272-2).
• Applicability to a wide range of matrices: foods, feed, environmental swabs and primary production samples; guidance for selecting detection vs. direct-plating strategies remains aligned with Part 1 (detection method).
• Requirements for confirmation steps, reporting formats (CFU per unit), and laboratory quality assurance/verification when implementing molecular confirmation alongside culture-based results.

Typical use and users

Used by food and feed microbiology laboratories, public health and regulatory testing laboratories, quality assurance departments in food producers, contract testing laboratories and research groups working on Campylobacter enumeration and identification. The amendment supports laboratories that wish to integrate molecular confirmation into routine colony-count workflows or that require updated media performance verification.

Related standards

ISO 10272 is a multi-part series. Key related documents include ISO 10272-1:2017 (Part 1: Detection method) and the original ISO 10272-2:2017 (Part 2: Colony-count technique). Both Part 1 and Part 2 have corresponding Amendment 1 documents published in 2023 which add molecular confirmation approaches and media performance updates. For implementation, laboratories typically refer to both Part 1 (detection/enrichment guidance) and Part 2 (enumeration and colony-count procedures).

Keywords

Campylobacter, colony-count, enumeration, food microbiology, molecular confirmation, PCR, media performance testing, thermotolerant Campylobacter, ISO 10272-2, amendment 2023.

FAQ

Q: What is this standard?

A: ISO 10272-2:2017/Amd 1:2023 is Amendment 1 (2023) to Part 2 of the ISO 10272 series, which updates the colony-count technique for enumeration of Campylobacter spp. by adding molecular confirmation/identification methods and revising media performance testing.

Q: What does it cover?

A: It covers additions and revisions to the colony-count (enumeration) procedure for Campylobacter spp., specifically methods for molecular confirmation/identification of thermotolerant Campylobacter and updated procedures and acceptance criteria for testing the performance of culture media used in enumeration. The underlying scope (applicability to food, feed and relevant environmental samples) remains the same as ISO 10272-2:2017.

Q: Who typically uses it?

A: Food and feed testing laboratories, public health labs, regulatory laboratories, contract testing organisations, and industry QA/QC teams that perform Campylobacter enumeration and that may integrate molecular confirmation methods into their workflows.

Q: Is it current or superseded?

A: This document is a published amendment (2023) to the 2017 Part 2 standard — it supplements and updates ISO 10272-2:2017 rather than fully replacing it. The amended package (ISO 10272-2:2017 together with Amd 1:2023) is the current normative text for Part 2. Publication of the amendment occurred in January 2023 (with some national adoptions dated early February 2023).

Q: Is it part of a series?

A: Yes — ISO 10272 is a series for Campylobacter methods in the food chain. Part 1 addresses detection (ISO 10272-1:2017, with its own 2023 amendment) and Part 2 covers the colony-count technique (this document). Laboratories implementing Campylobacter testing normally consult both parts.

Q: What are the key keywords?

A: Campylobacter, enumeration, colony-count, food microbiology, molecular confirmation, media performance, thermotolerant Campylobacter, ISO 10272-2, amendment 2023.