ISO 10519-2015 amd1-2025 PDF

Full title and description

ISO 10519:2015/Amd 1:2025 — Rapeseed — Determination of chlorophyll content — Spectrometric method — Amendment 1: Preparation of the calibration curve to determine the k factor. This amendment updates the 2015 spectrometric method by giving the procedure for preparing the calibration curve used to determine the k factor in chlorophyll measurements on rapeseed.

Abstract

ISO 10519:2015 specifies a spectrometric method for determining the chlorophyll content of rapeseed (not applicable to chlorophyll in oils). Amendment 1 (2025) adds details on preparation of the calibration curve to determine the k factor used in the calculation of chlorophyll content.

General information

• Status: Published.
• Publication date: 07 April 2025 (Amendment 1:2025).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 67.200.20 (Oilseeds).
• Edition / version: Amendment to ISO 10519:2015 — third edition (2015) with Amendment 1:2025 (Edition 3, amendment 2025).
• Number of pages: 2 (amendment document).

Scope

This amendment provides the procedure for preparing the calibration curve used to determine the k factor in the spectrometric determination of chlorophyll content of rapeseed. It is intended to be used together with ISO 10519:2015 and does not change the fundamental scope of the original method (which excludes determination of chlorophyll in oils).

Key topics and requirements

• Preparation and use of a calibration curve specifically to determine the k factor for chlorophyll calculations in rapeseed samples.
• Principles of the spectrometric measurement of chlorophyll and required instrumental considerations.
• Sample handling and preparation recommendations appropriate for rapeseed prior to spectrometric analysis.
• Calculation steps to convert spectrometric readings and the k factor into reported chlorophyll content.
• Requirements for documentation and reporting of results to ensure traceability and repeatability.

Typical use and users

Used by agricultural and seed-testing laboratories, research institutions studying oilseed quality, seed producers and breeders, quality-control laboratories in the oilseed supply chain, and regulatory or standards bodies that monitor seed quality. The amendment is applied by laboratories already using ISO 10519:2015 to improve calibration and measurement consistency.

Related standards

ISO 10519:2015 is the parent document; Amendment 1:2025 modifies and is to be used together with the 2015 edition. National and regional adoptions / identical publications (for example UNE/EN adoptions and national variants) exist for application in specific countries. The earlier ISO 10519 versions (1992, 1997) were superseded by the 2015 edition.

Keywords

rapeseed, oilseeds, chlorophyll, spectrometric method, calibration curve, k factor, ISO 10519, amendment 2025, seed testing, analytical method

FAQ

Q: What is this standard?

A: ISO 10519:2015 is an international standard that specifies a spectrometric method for determining chlorophyll content in rapeseed; ISO 10519:2015/Amd 1:2025 is an amendment that adds the procedure for preparing the calibration curve to determine the k factor.

Q: What does it cover?

A: It covers the spectrometric measurement procedure for chlorophyll in rapeseed, and the 2025 amendment covers how to prepare the calibration curve used to determine the k factor required in the chlorophyll calculation. The method is not applicable to determining chlorophyll in oils.

Q: Who typically uses it?

A: Accredited testing laboratories, seed quality and certification bodies, seed producers and breeders, research laboratories, and any organization needing standardized, reproducible chlorophyll measurements on rapeseed.

Q: Is it current or superseded?

A: The 2015 edition remains the current base standard and was updated by Amendment 1 published on 07 April 2025; the amendment is published and current and should be used together with ISO 10519:2015.

Q: Is it part of a series?

A: ISO 10519 is part of the broader ISO/TC 34/SC 2 work on oilseeds and related methods; it is specific to rapeseed chlorophyll determination and sits alongside other analytical standards for oilseeds and oilseed products.

Q: What are the key keywords?

A: Rapeseed, chlorophyll, spectrometric method, calibration curve, k factor, oilseeds, ISO 10519, amendment 2025.