ISO 10519-2015 PDF

Full title and description

ISO 10519:2015 — Rapeseed — Determination of chlorophyll content — Spectrometric method. This International Standard specifies a laboratory spectrometric procedure for extracting and measuring chlorophyll in rapeseed (reported as mg/kg chlorophyll a); it is not applicable to chlorophyll determination in oils.

Abstract

ISO 10519:2015 defines a solvent‑extraction/spectrometric method that covers sample preparation, extraction, absorbance measurement (with baseline correction) and calculation of chlorophyll content in rapeseed. The method is intended for routine quality control, research and inter‑laboratory comparability for oilseed material.

General information

• Status: Published (active international standard; reviewed and maintained by ISO/TC 34/SC 2).
• Publication date: 16 September 2015 (ISO edition 3, 2015).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 67.200.20 — Oilseeds.
• Edition / version: Edition 3 (2015). An amendment addressing calibration curve preparation (Amd 1) was published in 2025.
• Number of pages: 6 pages (main standard document).

Scope

This standard specifies a spectrometric method for determining the chlorophyll content of rapeseed (expressed as mg/kg chlorophyll a). It covers reduction of laboratory sample to test sample, drying where required, solvent extraction of ground seed, spectrometric measurement with baseline correction and calculation of results. It explicitly excludes determination of chlorophyll in oils.

Key topics and requirements

• Principle: solvent extraction of ground rapeseed followed by absorbance measurement and baseline correction to obtain a chlorophyll‑related absorbance value.
• Sample preparation: reduction of laboratory sample per ISO 664; if moisture >10 % (m/m), drying (e.g., 45 °C) to stabilise chlorophyll; grinding to obtain representative test portions.
• Extraction: use of an anhydrous organic solvent or solvent mixture to extract chlorophyll from a measured test portion (typical solvent volumes and ratios are specified in the method).
• Spectrometric measurement: absorbance readings at specified wavelengths (commonly 665 nm with supporting readings at 705 nm and 625 nm for baseline correction), optical path length and instrument performance requirements.
• Calculation: corrected absorbance (example form A_corr = A665 − (A705 + A625)/2) and conversion to mg/kg with a k factor and the sample/volume/path length variables (standard gives the calculation formula).
• Precision and reporting: repeatability and reproducibility performance criteria, and reporting requirements including method identification, sampling reference, moisture basis (if applicable), and any deviations.

Typical use and users

Laboratories and quality‑control units in oilseed processing, seed testing laboratories, agricultural research organisations and inter‑laboratory validation programmes use this standard when quantifying chlorophyll in rapeseed lots for quality assessment, breeding trials, regulatory testing and commercial trading.

Related standards

Normative references and related ISO standards used with ISO 10519 include ISO 664 (Oilseeds — Reduction of laboratory sample to test sample), ISO 665 (Determination of moisture and volatile matter content) and ISO 648 (Laboratory glassware — Single‑volume pipettes). National/adopted versions (for example EN/SS/BS adoptions) may be available.

Keywords

rapeseed, canola, chlorophyll, spectrometric method, oilseeds, chlorophyll a, extraction, ISO 10519, quality control.

FAQ

Q: What is this standard?

A: ISO 10519:2015 is an international standard that specifies a laboratory spectrometric method for determining the chlorophyll content of rapeseed (reported as mg/kg chlorophyll a).

Q: What does it cover?

A: It covers sample reduction, conditioning (moisture/drying), grinding, solvent extraction, spectrometric absorbance measurements (with baseline correction), calculation of chlorophyll concentration and reporting requirements; it does not apply to chlorophyll measurement in oils.

Q: Who typically uses it?

A: Oilseed testing laboratories, processing plant QC labs, seed technologists, researchers and organisations involved in seed quality assessment and trade use the method for routine testing and inter‑laboratory comparisons.

Q: Is it current or superseded?

A: The 2015 edition (Edition 3) is the current published version; earlier editions (1992, 1997) were withdrawn when the 2015 edition was published. An amendment (ISO 10519:2015/Amd 1) addressing calibration curve preparation was published in 2025. Users should check for any further amendments or confirmations before relying on the procedure.

Q: Is it part of a series?

A: ISO 10519 is a stand‑alone method for chlorophyll in rapeseed but sits within ISO/TC 34 work on oilseeds and may be referenced alongside other oilseed testing standards (for sampling, moisture determination, etc.). National/adopted versions (EN, BS, SS, etc.) may exist.

Q: What are the key keywords?

A: rapeseed, chlorophyll, spectrometric, extraction, oilseeds, chlorophyll a, ISO 10519.