ISO TR 17623-2015 PDF

Full title and description

ISO/TR 17623:2015 — Molecular biomarker analysis — SSR analysis of maize. This technical report gives recommended SSR (simple sequence repeat) markers and a practical workflow for reliable SSR analysis of maize for purposes such as hybrid conformity testing, molecular fingerprinting and variety identity checks.

Abstract

The document lists validated SSR markers and describes key procedural steps (sample preparation, DNA extraction, PCR amplification and product detection) and marker-specific details (allele size ranges, primer information and validation notes) to promote reproducible SSR results across laboratories. The methods and markers are intended for use in testing hybrid conformity, variety fingerprinting and identity verification.

General information

• Status: Published / confirmed (Technical Report in force).
• Publication date: May 8, 2015 (edition 1).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 67.050 — General methods of tests and analysis for food products.
• Edition / version: Edition 1 (2015); confirmed in a 5-year review.
• Number of pages: 6 pages (concise technical report).

Scope

ISO/TR 17623:2015 specifies a set of SSR markers and provides a recommended laboratory workflow for SSR analysis in maize. It is intended to harmonize marker choice and basic experimental steps so that results (allele calls and fingerprints) are comparable between laboratories for applications including hybrid conformity testing, variety identification and molecular fingerprinting in breeding and seed quality control.

Key topics and requirements

• List of recommended SSR markers for maize, with allele size ranges and marker characteristics.
• Guidance on sample preparation (seed/leaf sampling and grinding) to ensure representative DNA input.
• Recommended DNA-extraction approaches and notes on compatibility with downstream PCR.
• PCR amplification conditions and recommendations (including primer information and thermocycling guidance) to standardize amplification.
• Detection and sizing of PCR products (capillary electrophoresis / fluorescence detection) and guidance to produce comparable allele-calling.
• Notes on marker validation, intra-laboratory checks and use of public maize genomic resources for reference alleles.

Typical use and users

Primary users are plant molecular laboratories, seed testing and certification bodies, maize breeders, variety registration authorities and commercial seed companies. The report is used to harmonize marker sets and laboratory practices for routine identity testing, breeding program fingerprinting and regulatory conformity checks.

Related standards

ISO/TR 17623:2015 was developed under ISO/TC 34/SC 16 (food products — molecular methods). Related documents include other ISO guidance and methods on molecular biomarker analysis developed by ISO/TC 34 and national seed-testing standards that cover genetic identification and variety testing methods. Users should consult the ISO catalogue for the latest related publications from ISO/TC 34/SC 16.

Keywords

SSR, simple sequence repeat, microsatellite, maize, Zea mays, molecular biomarker, variety identification, hybrid conformity, PCR, capillary electrophoresis.

FAQ

Q: What is this standard?

A: ISO/TR 17623:2015 is a technical report that recommends SSR markers and a harmonized workflow for SSR-based molecular analysis of maize to support fingerprinting and identity/conformity testing.

Q: What does it cover?

A: It covers selection of SSR markers for maize and practical laboratory steps (sampling, DNA extraction, PCR conditions and product detection/sizing) and includes marker-specific information to help laboratories produce reproducible allele calls.

Q: Who typically uses it?

A: Molecular diagnostic and research labs, seed testing and certification agencies, maize breeders, seed companies and regulatory bodies concerned with variety identity and hybrid conformity.

Q: Is it current or superseded?

A: The report was published on May 8, 2015 (Edition 1) and was maintained through ISO’s review process (confirmed during routine review). Users should check ISO’s catalogue for any subsequent confirmations or revisions.

Q: Is it part of a series?

A: It was developed under ISO/TC 34/SC 16 (methods for food products and related molecular methods). While not a multi-part standard series itself, it complements other ISO molecular-methods guidance from the same technical committee.

Q: What are the key keywords?

A: SSR, microsatellite, maize, molecular marker, variety identification, hybrid conformity, PCR, allele sizing.