ISO TR 22814-2020 PDF

Full title and description

ISO/TR 22814:2020 — Good practice for dynamic light scattering (DLS) measurements. This technical report provides practical, application‑focused guidance to plan, perform and interpret DLS measurements (including sample preparation, instrument verification and data‑quality assessment) for particles, emulsions and fine bubbles dispersed in liquids.

Abstract

The report complements ISO 22412 by going beyond treatment of measurement artefacts to give hands‑on recommendations for experiment planning, obtaining required sample information, preparing samples, verifying instrument function, assessing data quality and interpreting results for solids, emulsions and bubbles. The emphasis is on practical steps to obtain reliable DLS results rather than on detailed theoretical development.

General information

• Status: Withdrawn (withdrawal recorded in ISO lifecycle; withdrawal activity began October 3, 2025).
• Publication date: April 2020 (ISO publication date: 2020‑04; some catalogue entries give 14 April 2020).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 19.120 — Particle size analysis; Particle characterization (ISO/TC 24/SC 4).
• Edition / version: Edition 1 (2020).
• Number of pages: 21 pages (ISO edition). Note: some national/adopted publications (PD/BSI, DIN listings) show extended packaging/cover pages and list 34 pages for their published PDF.

Scope

Provides practical guidance for performing and interpreting dynamic light scattering (DLS) measurements, with focus on experimental planning, sample information and preparation, verification of instrument performance, assessment of data quality and interpretation of results. Applies to measurements of solid particles, emulsions and fine bubbles dispersed in liquids and is intended to complement (not replace) normative methods such as ISO 22412.

Key topics and requirements

• Guidance on deciding when DLS is an appropriate technique (sample requirements and limitations).
• Recommendations for sample preparation to minimise contamination, aggregation and multiple scattering (filtration, dilution, handling).
• Instrument verification and checks to confirm proper functioning and optical alignment prior to measurement.
• Practical measurement considerations (scattering angle, instrument configuration, homodyne/heterodyne approaches) that influence result reliability.
• Data quality assessment methods and interpretation advice, including ways to detect and account for artefacts and multimodal distributions.
• Specific notes on measuring solids, emulsions and bubbles and on limitations with concentrated suspensions (multiple scattering, interactions).

Typical use and users

Used by laboratory scientists and technicians performing particle‑size analysis, researchers in colloid and formulation science, QA/QC personnel in pharmaceuticals and cosmetics, instrumentation engineers and manufacturers of light‑scattering equipment, and standards developers seeking practical measurement guidance. The report is primarily practical rather than normative and is consulted where users need actionable steps to improve DLS measurement quality.

Related standards

Directly complements ISO 22412 (Particle size analysis — Dynamic light scattering). Users should consult ISO 22412 for the normative measurement procedures and use ISO/TR 22814 for supplementary good‑practice guidance. Related ISO work comes from ISO/TC 24/SC 4 (Particle characterization).

Keywords

Dynamic light scattering; DLS; particle size analysis; sample preparation; instrument verification; data quality; emulsions; fine bubbles; photon correlation spectroscopy; quasi‑elastic light scattering; ISO TR 22814.

FAQ

Q: What is this standard?

A: ISO/TR 22814:2020 is a Technical Report (good‑practice guidance) titled "Good practice for dynamic light scattering (DLS) measurements" published by ISO in April 2020.

Q: What does it cover?

A: It covers practical steps for planning, performing and interpreting DLS experiments — including sample information and preparation, instrument checks, measurement considerations, data‑quality assessment and interpretation — for particles, emulsions and fine bubbles in liquids. It is intended to supplement the normative procedures in ISO 22412.

Q: Who typically uses it?

A: Researchers, laboratory analysts, instrument manufacturers, and quality assurance staff who perform or evaluate DLS measurements and need practical, hands‑on guidance to obtain reliable results.

Q: Is it current or superseded?

A: The ISO record shows ISO/TR 22814:2020 as Withdrawn; withdrawal activity is recorded in the ISO lifecycle (withdrawal activity began 3 October 2025). Users requiring normative measurement methods should follow the current edition of ISO 22412 (and related ISO documents).

Q: Is it part of a series?

A: It is a standalone ISO Technical Report produced under ISO/TC 24/SC 4 (particle characterization) and is intended as a complementary guidance document to ISO 22412 (DLS measurement standard). It is not a multi‑part numbered series but sits with other ISO guidance and standards for particle size analysis.

Q: What are the key keywords?

A: Dynamic light scattering, DLS, particle size analysis, sample preparation, instrument verification, data quality, emulsions, bubbles, ISO TR 22814.