ISO TS 5354-2-2024 PDF

Full title and description

ISO/TS 5354-2:2024 — Molecular biomarkers — Detection of DNA in cotton used for textile production — Part 2: Overview of target sequences for use in polymerase chain reaction (PCR)-based detection methods for cotton genetically modified (GM) events. This technical specification provides a compiled list of DNA target sequences intended to support PCR-based screening for the presence of genetically modified cotton material in textile raw materials and derived products.

Abstract

This document supplies an authoritative list of target sequences that laboratories and testing bodies can use to screen for currently known GM cotton events and for events that contain the same DNA sequences. It applies where sufficiently high‑quality, PCR‑amplifiable DNA can be extracted from cottonseed, cotton leaf, cotton fibre and cotton fibre‑derived materials; methods for extraction are referenced in Part 1 of ISO 5354. Sampling is explicitly outside the scope of this part.

General information

• Status: Published
• Publication date: April 2024 (published 19 April 2024 in commercial catalogues and ISO listing shows April 2024).
• Publisher: International Organization for Standardization (ISO)
• ICS / categories: 59.080.01 (Textiles in general)
• Edition / version: Edition 1 (2024-04)
• Number of pages: 13 (ISO bibliographic record; alternative commercial listings may show different page counts for packaged/PD editions).

Key bibliographic and lifecycle metadata are recorded on the ISO bibliographic entry for this technical specification; commercial distributors list the same April 2024 publication timeframe.

Scope

ISO/TS 5354-2:2024 is limited to providing an overview of target DNA sequences usable in PCR‑based detection of genetically modified cotton events. It is applicable to cottonseed, cotton leaf, cotton fibre and cotton fibre‑derived materials from which sufficiently high‑quality PCR‑amplifiable DNA can be obtained. The document does not specify sampling procedures; it cross‑references ISO 5354‑1 for DNA extraction methods and notes external guidance for screening foodstuffs.

Key topics and requirements

• Compiled list of target sequences recommended for PCR screening of known GM cotton events.
• Guidance to enable detection of GM events that contain the same target sequences (enables broad screening strategy rather than event‑by‑event assays).
• Cross‑reference to ISO 5354‑1 for validated DNA extraction methods from cotton materials.
• Applicability limited to samples yielding PCR‑amplifiable DNA; sampling protocols are out of scope.
• Intended use in regulated testing, certification and supply‑chain screening workflows; laboratories may need to transition from previously used methods to the ISO approach.

Typical use and users

Primary users are analytical laboratories performing GMO screening of textile raw materials and textile‑derived products, accreditation bodies, certification schemes (e.g., textile sustainability and non‑GM claims), regulatory agencies, customs laboratories, and research organizations studying cotton biotechnology or textile forensics. The specification is used to select PCR targets for routine screening and confirmatory testing where DNA of sufficient quality can be obtained from fibre or other cotton materials. Laboratories implementing or updating methods should be aware of timing for transition from earlier practices (including IWA 32) to the ISO 5354 series.

Related standards

ISO/TS 5354-2:2024 is part of the ISO 5354 series and is directly related to: ISO 5354‑1 (Part 1: Extraction of DNA from cotton seed and raw materials derived therefrom). The document also references sampling guidance (ISO 1130:1975 and ASTM D1441 series) and complementary regional guidance for foodstuff screening (e.g., CEN/TS 16707). IWA 32:2019 (the earlier guidance) has been withdrawn/updated in the ISO series and stakeholders have been advised about the transition to the ISO 5354 family.

Keywords

molecular biomarkers; PCR; DNA detection; cotton; genetically modified (GM) events; target sequences; DNA extraction; textile testing; GMO screening; ISO 5354.

FAQ

Q: What is this standard?

A: ISO/TS 5354-2:2024 is a technical specification that lists DNA target sequences for PCR‑based detection methods intended to screen for genetically modified cotton events in cotton raw materials and textile‑derived products.

Q: What does it cover?

A: It provides an overview of target DNA sequences to be used in PCR screening; it does not provide sampling procedures and applies only when sufficiently high‑quality PCR‑amplifiable DNA can be extracted from the sample. Extraction methods are covered in ISO 5354‑1.

Q: Who typically uses it?

A: Analytical laboratories (including customs and regulatory labs), certification and conformity assessment bodies, textile manufacturers and supply‑chain auditors, and research institutions involved in GMO detection and textile forensics.

Q: Is it current or superseded?

A: As of its publication in April 2024 it is the current ISO technical specification for target sequences in cotton GMO PCR screening; it forms part of the update that replaced/updated previous guidance such as IWA 32:2019 and is intended to be adopted by laboratories using ISO 5354 series methods. Stakeholders should follow official ISO announcements and national accreditation guidance for transition timing.

Q: Is it part of a series?

A: Yes — it is Part 2 of the ISO 5354 series. Part 1 (ISO 5354‑1) covers DNA extraction from cotton seed and raw materials; Part 2 provides the overview of PCR target sequences.

Q: What are the key keywords?

A: PCR, DNA detection, cotton, genetically modified (GM) events, target sequences, molecular biomarkers, textile testing, ISO 5354.