Overview

ASTM E211-82(2022) - Standard Specification for Cover Glasses and Glass Slides for Use in Microscopy establishes the required properties and test methods for glass covers and slides intended for routine microscopy. Developed by ASTM International, this specification supports quality, reproducibility, and global compatibility in the supply and use of microscope glassware. It details criteria such as dimensions, planeness, parallelism, corrosion resistance, clarity, wettability, index of refraction, and workmanship. The use of this standard ensures laboratory glassware meets consistent international expectations for reliable microscopical analysis, enhancing performance in research and diagnostic settings.

Key Topics

Material Quality
- Cover glasses and slides must be made from colorless, transparent glass.
- The glass must be free from pits, bubbles, cloudiness, or defects that may affect performance.
- Refractive index specifications are provided for both covers and slides to ensure optical clarity.
Dimensions and Tolerances
- Several standard sizes for square, rectangular, and circular cover glasses.
- Glass slide sizes and thickness options are provided, with strict tolerances to ensure compatibility with most microscopes.
- Specialized dimensions for different microscopy techniques, including darkfield, interference, embryology, and petrography.
Performance Requirements
- Planeness and parallelism criteria ensure consistent focal planes in microscopic imaging.
- Glasses and slides must resist corrosion and withstand boiling, ensuring durability during laboratory procedures.
- Clarity is verified by visual inspection, while wettability tests confirm even spreading of biological materials.
Packaging and Labeling
- Glassware must be packaged in a way that prevents contamination, dust intrusion, and breakage.
- Essential product information, such as size, thickness, manufacturer, and country of origin, must be clearly labeled on the packaging.

Applications

ASTM E211-82(2022) has practical applications across various laboratory and clinical environments, promoting reliable and consistent results in microscopy. Key uses include:

Medical Diagnostics
- Preparation of blood smears, tissue sections, and microbial samples for examination under the microscope.
Biological and Biomedical Research
- Handling and observation of cells, tissues, and organisms.
Industrial and Quality Control Laboratories
- Analysis and validation of materials, such as in pharmaceuticals, environmental monitoring, and material sciences.
Education and Training
- Standardizing teaching materials and laboratory practice for students and trainees.

Laboratories, manufacturers, and suppliers implementing this ASTM standard benefit from increased reliability and international acceptance of their glassware. By adhering to the defined specifications, users can minimize variability, reduce sample preparation errors, and uphold the highest standards for microscopy quality.

Related Standards

Organizations seeking comprehensive assurance in laboratory glassware may also consult these related standards:

ASTM E920 - Standard Specification for Laboratory Glass Thermometers
ASTM E438 - Standard Specification for Glasses in Laboratory Apparatus
ISO 8255 - Microscopes - Slides and Cover Glasses - Dimensions

These documents, along with ASTM E211-82(2022), contribute to a cohesive framework for laboratory glassware quality and compatibility, supporting precision and safety in microscopy applications.

Keywords: microscopy, cover glass, glass slides, laboratory, standard, ASTM E211, optical clarity, laboratory glassware, specimen preparation.

Overview

Key Topics

Material Quality
- Cover glasses and slides must be made from colorless, transparent glass.
- The glass must be free from pits, bubbles, cloudiness, or defects that may affect performance.
- Refractive index specifications are provided for both covers and slides to ensure optical clarity.
Dimensions and Tolerances
- Several standard sizes for square, rectangular, and circular cover glasses.
- Glass slide sizes and thickness options are provided, with strict tolerances to ensure compatibility with most microscopes.
- Specialized dimensions for different microscopy techniques, including darkfield, interference, embryology, and petrography.
Performance Requirements
- Planeness and parallelism criteria ensure consistent focal planes in microscopic imaging.
- Glasses and slides must resist corrosion and withstand boiling, ensuring durability during laboratory procedures.
- Clarity is verified by visual inspection, while wettability tests confirm even spreading of biological materials.
Packaging and Labeling
- Glassware must be packaged in a way that prevents contamination, dust intrusion, and breakage.
- Essential product information, such as size, thickness, manufacturer, and country of origin, must be clearly labeled on the packaging.

Applications

ASTM E211-82(2022) has practical applications across various laboratory and clinical environments, promoting reliable and consistent results in microscopy. Key uses include:

Medical Diagnostics
- Preparation of blood smears, tissue sections, and microbial samples for examination under the microscope.
Biological and Biomedical Research
- Handling and observation of cells, tissues, and organisms.
Industrial and Quality Control Laboratories
- Analysis and validation of materials, such as in pharmaceuticals, environmental monitoring, and material sciences.
Education and Training
- Standardizing teaching materials and laboratory practice for students and trainees.

Related Standards

Organizations seeking comprehensive assurance in laboratory glassware may also consult these related standards:

ASTM E920 - Standard Specification for Laboratory Glass Thermometers
ASTM E438 - Standard Specification for Glasses in Laboratory Apparatus
ISO 8255 - Microscopes - Slides and Cover Glasses - Dimensions

These documents, along with ASTM E211-82(2022), contribute to a cohesive framework for laboratory glassware quality and compatibility, supporting precision and safety in microscopy applications.

Keywords: microscopy, cover glass, glass slides, laboratory, standard, ASTM E211, optical clarity, laboratory glassware, specimen preparation.

ASTM E211-82(2022) PDF

Технические детали

Abstract

Overview

Key Topics

Applications

Related Standards

Похожие стандарты

Abstract

Overview

Key Topics

Applications

Related Standards

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