Cosmetics. Microbiology. Detection of specified and non-specified microorganisms
Cosmetics. Microbiology. Detection of specified and non-specified microorganisms
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1 Scope This document gives general guidelines for the detection and identification of specified microorganisms in cosmetic products as well as for the detection and identification of other kinds of aerobic mesophilic non-specified microorganisms in cosmetic products . Microorganisms considered as specified in this document might differ from country to country according to national practices or regulations. Most of them considered as specified microorganisms include one or more of the following species: Pseudomonas aeruginosa , Escherichia coli , Staphylococcus aureus and Candida albicans . In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity, hydro-alcoholic products , extreme pH values, etc. The method described in this document is based on the detection of microbial growth in a non-selective liquid medium ( enrichment broth ) suitable to detect microbial contamination, followed by isolation of microorganisms on non-selective agar media. Other methods can be appropriate depending on the level of detection required. In this document specific indications are given for identification of
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