1 Scope
This document specifies requirements and recommendations to laboratories that perform
extraction of polymerase chain reaction (PCR) quality deoxyribonucleic acid (DNA)
from cottonseed , cotton leaf and raw material derived therefrom, that is sufficient for the purpose of PCR analysis.
This document is applicable to:
a) identifying cotton raw material from which PCR quality DNA can be extracted;
b) specifying a method for effective DNA extraction from cotton and cotton-derived raw
materials;
c) specifying the cotton-specific marker(s) to be used as controls for PCR amplification
of DNA.
A PCR result obtained from analysis of cottonseed , cotton leaf and to some extant raw materials derived therefrom can only indicate that it is not
derived from pure genetically modified organism (GMO)-derived cotton. Admixtures of
GMO-derived cotton cannot be detected for cotton fibre and cotton fibre-derived materials.
This document does not apply to bulk sampling of the seed, bale or processed fabric and yarn. A recommended sampling method is given in ISO 6497 [ 5 ] . General guidance for the sampling of bulk materials or for cotton-based products
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