GOST 32881-2014 PDF

Full title and description

GOST 32881-2014 — Продукты пищевые, продовольственное сырьё. Метод определения остаточного содержания нестероидных противовоспалительных лекарственных средств с помощью высокоэффективной жидкостной хроматографии с масс‑спектрометрическим детектором (Food products, food raw materials. Method for determination of the non‑steroidal anti‑inflammatory drug residue content by high performance liquid chromatography — mass spectrometry). Стандарт описывает методику ВЭЖХ‑МС/МС для количественного определения остатков НПВП в пищевых матрицах.

Abstract

This intergovernmental standard establishes a validated analytical procedure based on high‑performance liquid chromatography coupled with tandem mass spectrometric detection (HPLC‑MS/MS) for the determination of residues of non‑steroidal anti‑inflammatory drugs (NSAIDs) in selected foodstuffs and food raw materials (milk and dairy, meat and meat products, poultry). The method uses matrix‑matched calibration and internal standards, sample extraction and cleanup (including enzymatic hydrolysis and solid‑phase extraction where applicable), and defines metrological characteristics and quality control procedures. Measurement range is specified from 0.1 to 1000.0 µg/kg.

General information

• Status: Active / in force (introduced as an interstate standard and adopted as a national standard of the Russian Federation).
• Publication date: Approved by Rosstandart order 30 September 2014; date of compulsory introduction as national standard: 1 January 2016.
• Publisher: Interstate (Межгосударственный) standard adopted via the Federal Agency for Technical Regulation and Metrology (Rosstandart); published editions distributed by official standards publishers/collections.
• ICS / categories: 65.020.30; 65.120; 67.050; 67.120 (food analysis; general methods of tests and analysis for food products; meat and poultry sectors).
• Edition / version: Designation year 2014 (first introduction in 2016); amendments and corrections published in information indexes (IUS) and later updates (amendments noted in 2017, 2018, 2020 and an amendment published in IUS 9/2024).
• Number of pages: Approximately 20 pages (standard text and mandatory Annex A). Vendor/registry records list 20 pages (some reprints/editions list minor variations).

Scope

Applies to food products and food raw materials specifically in the parts of milk and dairy products, meat and meat products, and poultry products. Establishes a quantitative HPLC‑MS/MS procedure for determination of residual concentrations of a range of NSAIDs in these matrices within the analytical range 0.1–1000.0 µg/kg, and details sample preparation, chromatographic/ MS conditions, calibration, calculation of results, and metrological performance characteristics.

Key topics and requirements

• Analytical technique: high‑performance liquid chromatography with tandem mass spectrometric detection (HPLC‑MS/MS / ВЭЖХ‑МС/МС) for selective, sensitive quantitation of NSAIDs.
• Target matrices: milk, dairy products, meat (including red meat) and meat products, and poultry products; provision for matrix‑matched calibration.
• Sample preparation: procedures include enzymatic hydrolysis (e.g., β‑glucuronidase) where conjugates are present, solvent extraction, concentration, and cleanup (solid‑phase extraction cartridges and related steps).
• Calibration and quantitation: use of internal standards (including isotopically labelled analogues where specified), matrix calibration curves with a minimum number of concentration levels, and peak area‑based quantitation (MRM transitions).
• Performance characteristics: declared measurement range 0.1–1000 µg/kg, repeatability/reproducibility controls, uncertainty estimation, limit of quantification guidance and stability control procedures (including mandatory Annex on stability control).
• Quality assurance: requirements for control of extraction recovery, internal standard performance, instrument parameters, and documentation/formatting of results.
• Safety and environmental conditions: laboratory environmental limits, equipment and reagent specifications, and standard laboratory safety references (normative links to general safety and reagent standards).

Typical use and users

Primary users are accredited food‑testing laboratories, veterinary and sanitary control authorities, food producers and quality departments, research institutes working on pesticide/ veterinary drug residue monitoring, and conformity assessment bodies performing official controls and routine monitoring of NSAID residues in food chains. Laboratories adopt the method for official control, compliance testing, and research where sensitive, selective quantification of NSAID residues is required.

Related standards

The standard references and is used together with general standards on standardization and measurement methods such as GOST 1.0‑92 and GOST 1.2‑2009 (principles and rules of interstate standardization), GOST/ISO guidance on measurement accuracy (ISO/GOST 5725 series), and multiple laboratory practice and reagent standards (for example standards on reagents, laboratory glassware and sampling methods for milk and meat). It is also listed among methods relevant to EAEU technical regulations on safety of fish and fish products and other food safety regulatory lists.

Keywords

GOST 32881-2014; NSAIDs; non‑steroidal anti‑inflammatory drugs; food analysis; HPLC‑MS/MS; VЭЖХ‑MS/MS; method of determination; milk; dairy; meat; poultry; residue; matrix‑matched calibration; internal standards.

FAQ

Q: What is this standard?

A: GOST 32881‑2014 is an intergovernmental (Межгосударственный) analytical standard that specifies a validated HPLC‑MS/MS method for the determination of residues of non‑steroidal anti‑inflammatory drugs in selected food products and raw materials.

Q: What does it cover?

A: It covers sample preparation, extraction and cleanup procedures, chromatographic and mass‑spectrometric measurement conditions, calibration (matrix‑matched and internal standards), result calculation, quality control and metrological characteristics for measurement of NSAID residues in milk, dairy, meat and poultry matrices (range 0.1–1000 µg/kg).

Q: Who typically uses it?

A: Accredited food testing and veterinary laboratories, regulatory control agencies, food industry quality/control departments, and research institutions involved in residue monitoring and food safety testing use this standard.

Q: Is it current or superseded?

A: As published it is an active (in‑force) interstate standard adopted for use as a national standard in the Russian Federation; amendments and corrections have been issued (summarized in IUS entries and vendor registries), the standard text includes updates up to notices published in information indexes (including amendments recorded through 2024). Users should check the most recent information index or national registry for the latest amendments before use.

Q: Is it part of a series?

A: It is a standalone method standard within the set of food‑analysis standards but is linked by normative references to general standards on standardization, laboratory practice and measurement accuracy (GOST 1.0‑92, GOST 1.2‑2009, ISO/GOST 5725 series, and multiple reagent and glassware standards).

Q: What are the key keywords?

A: Key keywords include NSAIDs, HPLC‑MS/MS, food products, milk, meat, poultry, residue analysis, internal standard, matrix‑matched calibration, method validation.