ISO 10272-1-2017 PDF

Full title and description

ISO 10272-1:2017 — Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method. This International Standard specifies a horizontal procedure for the detection of thermotolerant Campylobacter spp. in food, feed, environmental and primary production samples using enrichment and/or direct plating approaches.

Abstract

ISO 10272-1:2017 defines three detection procedures (enrichment in Bolton broth, enrichment in Preston broth, and direct plating) and the associated isolation and confirmation steps for presumptive Campylobacter colonies. It is intended for use on products for human consumption and animal feeding, environmental samples from food/feed production and handling, and primary production samples such as animal faeces, dust and swabs.

General information

• Status: Published (International Standard; confirmed 2022).
• Publication date: June 2017 (2017-06; published 28 June 2017 in ISO record).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 07.100.30 — Food microbiology.
• Edition / version: Edition 2 (2017).
• Number of pages: 24 pages (ISO published PDF length).

Scope

Specifies a horizontal method for the detection of Campylobacter spp. by enrichment or direct plating. Applicable sample types include foods intended for human consumption, animal feeding stuffs, environmental samples from food and feed production and handling, and primary production samples (for example faeces, dust and swabs). The standard covers sample preparation, choice of detection procedure (A, B or C), selective enrichment or direct plating, isolation on selective agar, and confirmation of presumptive colonies.

Key topics and requirements

• Three detection procedures: Procedure A — selective enrichment in Bolton broth for samples with low Campylobacter numbers and low background flora; Procedure B — selective enrichment in Preston broth for samples with low Campylobacter numbers and high background flora; Procedure C — direct plating for samples with high Campylobacter levels.
• Typical incubation regimes: Bolton broth pre-enrichment/incubation sequence (microaerobic 37 °C for 4–6 h then 41.5 °C for ~44 h); Preston broth enrichment (microaerobic 41.5 °C for ~24 h); direct plates incubated at ≈41.5 °C under microaerobic conditions.
• Isolation on selective solid media (for example modified Charcoal Cefoperazone Deoxycholate agar — mCCDA — and an alternative selective medium) followed by examination of presumptive colonies.
• Confirmation workflow: microscopic/motility and morphological checks, oxidase/aerobic growth tests and optional biochemical or molecular identification; amendment 2023 includes methods for molecular confirmation and identification of thermotolerant Campylobacter spp.
• Requirements and guidance for culture media preparation, performance testing and quality control, with cross‑references to other ISO standards on media preparation and test sample handling.

Typical use and users

Used by public health and food safety laboratories, industrial quality control laboratories in food production (including poultry and dairy), contract testing laboratories, research institutions studying foodborne Campylobacter, and regulatory agencies conducting surveillance and outbreak investigations. The method is intended to support presence/absence testing as well as screening prior to enumeration (Part 2).

Related standards

ISO 10272 is a multi‑part series: Part 1 is the detection method (this document) and Part 2 covers the colony‑count (enumeration) technique (ISO 10272-2:2017). Other related standards referenced for sample handling and media include ISO 11133 (preparation and performance testing of culture media), ISO 6887 (sample preparation, initial suspension and decimal dilutions) and ISO 7218 (general requirements and guidance for microbiological examinations). Amendment 1 (2023) applied to both Part 1 and Part 2 to add molecular confirmation options and media performance changes.

Keywords

Campylobacter, food microbiology, detection method, enrichment, Bolton broth, Preston broth, mCCDA, microaerobic incubation, confirmation, ISO 10272, food safety.

FAQ

Q: What is this standard?

A: ISO 10272-1:2017 is the ISO horizontal standard that specifies methods for detection of Campylobacter spp. in food, feed, environmental and primary production samples (Part 1 — detection method).

Q: What does it cover?

A: It covers sample preparation, three alternative detection procedures (enrichment in Bolton or Preston broth, or direct plating), isolation on selective agars, and procedures for confirmation of presumptive Campylobacter colonies. The 2023 amendment added molecular confirmation/identification options and some media performance updates.

Q: Who typically uses it?

A: National and regional public health laboratories, food industry quality/control labs, contract testing laboratories, researchers, and regulators performing surveillance, compliance testing and outbreak investigations.

Q: Is it current or superseded?

A: The 2017 edition (Edition 2) is published and was confirmed in 2022. An Amendment 1 (2023) was published to include molecular confirmation methods and media/performance updates. Users should ensure they apply any applicable amendments when implementing the method.

Q: Is it part of a series?

A: Yes — ISO 10272 is a series. Part 1 is the detection method (this document) and Part 2 is the colony‑count (enumeration) technique (ISO 10272-2:2017). A former Part 3 (semi‑quantitative) exists as a withdrawn technical specification.

Q: What are the key keywords?

A: Campylobacter, detection, enrichment, Bolton broth, Preston broth, mCCDA, microaerobic incubation, food microbiology, enumeration (Part 2), confirmation, molecular identification.