ISO 10272-1-2017 amd1-2023 PDF

Full title and description

ISO 10272-1:2017/Amd 1:2023 — Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method — Amendment 1: Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp., the use of a growth supplement in Preston broth and changes in the performance testing of culture media.

Abstract

Specifies a horizontal method for detection (by enrichment or direct plating) of Campylobacter spp. applicable to food and feed products, environmental samples in food/feed production and handling, and primary production samples such as faeces, dust and swabs. Amendment 1 (2023) adds molecular confirmation and identification methods for thermotolerant Campylobacter spp., permits use of a growth supplement in Preston broth, and updates performance-testing requirements for culture media.

General information

• Status: Published.
• Publication date: Amendment published January 25, 2023 (applies to ISO 10272-1:2017).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 07.100.30 — Food microbiology.
• Edition / version: Edition 2 (ISO 10272-1:2017) with Amendment 1:2023.
• Number of pages: 26 (amendment document length).

Scope

This document specifies validated laboratory procedures for the detection of Campylobacter spp. in food, feed and related environmental samples using enrichment or direct plating. The amendment extends the standard to include molecular confirmation/identification methods for thermotolerant Campylobacter species, clarifies use of growth supplements in Preston broth, and revises culture media performance testing. It is intended for use across the food chain from primary production to consumer-level sampling.

Key topics and requirements

• Detection options: enrichment-based and direct plating approaches for presumptive isolation of Campylobacter spp. from diverse sample matrices.
• Molecular confirmation and identification: addition of validated molecular methods for confirming thermotolerant Campylobacter spp. introduced by Amendment 1 (2023).
• Preston broth: allowance and instructions for use of a growth supplement in Preston enrichment broth to improve recovery.
• Culture media performance testing: updated criteria and procedures for verifying the performance of selective media used in the method.
• Sample applicability: food products for human consumption, animal feedingstuffs, environmental swabs and primary production samples (faeces, dust, etc.).
• Confirmation workflow: presumptive colony selection followed by biochemical and/or molecular confirmation as specified.

Typical use and users

Used by food testing and quality-control laboratories, public health and veterinary diagnostic labs, regulatory agencies, research laboratories, and food industry microbiologists for routine surveillance, outbreak investigation and compliance testing. The amendment is especially relevant to labs adopting molecular confirmation techniques or validating modified enrichment media.

Related standards

This is Part 1 of the ISO 10272 series. Relevant related documents include ISO 10272-2:2017 (Part 2: Colony-count/enumeration technique) and the corresponding Amendment 1:2023 for Part 2, which align enumeration and confirmation approaches across the series. Users should consult both parts when planning detection and enumeration workflows.

Keywords

Campylobacter, food microbiology, detection method, enrichment, Preston broth, molecular confirmation, thermotolerant Campylobacter, culture media performance, ISO 10272-1, amendment 2023.

FAQ

Q: What is this standard?

A: It is ISO 10272-1:2017 with Amendment 1:2023 — an international standard that defines laboratory methods for detecting Campylobacter spp. in food, feed and related samples, including new guidance for molecular confirmation and certain media amendments.

Q: What does it cover?

A: Detection by enrichment or direct plating, presumptive identification of colonies, confirmation (now including molecular methods), use of Preston broth with optional growth supplement, and requirements for culture media performance testing.

Q: Who typically uses it?

A: Food and feed testing laboratories, public-health and veterinary labs, regulatory authorities, diagnostic and research laboratories, and food industry QC/assurance teams.

Q: Is it current or superseded?

A: As of the amendment publication (25 January 2023) the 2017 edition plus Amendment 1:2023 is the current published document for Part 1; the amendment updates and extends the 2017 content rather than superseding the base standard. Users should confirm whether any later revisions or national adoptions apply in their jurisdiction.

Q: Is it part of a series?

A: Yes — it is Part 1 of the ISO 10272 series (detection and enumeration of Campylobacter spp.). Part 2 addresses enumeration (colony-count technique) and has a corresponding 2023 amendment.

Q: What are the key keywords?

A: Campylobacter, detection, enrichment, Preston broth, molecular confirmation, thermotolerant Campylobacter, culture media performance, food microbiology, ISO 10272-1:2017/Amd 1:2023.