ISO 10504-2013 PDF

Full title and description

ISO 10504:2013 — Starch derivatives — Determination of the composition of glucose syrups, fructose syrups and hydrogenated glucose syrups — Method using high-performance liquid chromatography. This International Standard specifies an HPLC procedure for measuring the major saccharides and related polyols in dextrose solutions and syrup products (glucose, maltose, maltotriose, fructose, sorbitol, mannitol, maltitol and malto‑oligosaccharides) and requires the use of a cation‑exchange column.

Abstract

ISO 10504:2013 describes a validated high‑performance liquid chromatography (HPLC) method for determining the composition of starch‑derived syrups and hydrogenated glucose syrups. The procedure covers sample preparation, chromatographic separation on a cation‑exchange resin column, detection (typically by differential refractometry), calculation of component percentages and reporting; an informative annex gives examples of standard solutions.

General information

• Status: Published; edition confirmed (current).
• Publication date: 2013-09 (Second edition, 2013).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 67.180.20 (Starch and derived products).
• Edition / version: Edition 2 (2013).
• Number of pages: 7 (official ISO pamphlet length; some national/adopted versions include additional front matter).

Core bibliographic and status information as listed in the ISO catalogue and national/adopted texts.

Scope

The standard specifies an HPLC method to determine the composition of dextrose solutions, glucose and fructose syrups, hydrogenated glucose syrups and related polyols (sorbitol, mannitol, maltitol). It is intended for analytical laboratories and industry quality control to quantify principal mono‑ and oligosaccharides and certain sugar alcohols in starch‑derived products. The method requires a cation‑exchange resin column and appropriate calibration/standard solutions.

Key topics and requirements

• Principle: HPLC separation on a cation‑exchange column with aqueous eluent and refractive index detection (thermostatted differential refractometer) or equivalent detection compatible with non‑derivatised sugars.
• Analytes covered: glucose, fructose, maltose, maltotriose, malto‑oligosaccharides and typical sugar alcohols (sorbitol, mannitol, maltitol).
• Column requirements: pre‑packed cation‑exchange resin (choice of counter‑ion and cross‑linking affects resolution); column conditioning and calibration are essential.
• Reagents and sample preparation: demineralized water for eluents, standard solutions for calibration, and sample demineralization/filtration as required for syrup matrices.
• Performance characteristics: procedures for calibration, calculation, precision and reporting are specified; an informative annex supplies example standard solutions.

Technical detail and mandatory requirements are set out in clauses covering principle, reagents, apparatus, procedure, calculation and precision.

Typical use and users

Used by analytical laboratories, food industry quality control units, starch processors, syrup producers and regulatory/inspection laboratories to verify product composition, label claims and process control. The method is suited for routine quality testing and inter‑laboratory comparisons when HPLC infrastructure and trained analysts are available.

Related standards

ISO 10504 references and is commonly used alongside other analytical standards for syrup and laboratory quality, notably ISO 5381 (Starch hydrolysis products — determination of water content — Modified Karl Fischer method) and ISO 3696 (Water for analytical laboratory use). The EN ISO adoption (EN ISO 10504:2015) is an identical regional adoption used in Europe.

Keywords

HPLC, cation‑exchange chromatography, glucose syrup, fructose syrup, hydrogenated glucose syrup, starch derivatives, sugar analysis, sorbitol, mannitol, maltitol, malto‑oligosaccharides, analytical method, ISO 10504.

FAQ

Q: What is this standard?

A: ISO 10504:2013 is an International Standard that defines an HPLC method for determining the composition of glucose and fructose syrups and hydrogenated glucose syrups (including certain polyols) produced from starch.

Q: What does it cover?

A: It covers the analytical principle, required reagents and apparatus, sample preparation, chromatographic procedure, calculation of component percentages, precision requirements and example standard solutions for calibration. The method emphasizes use of a cation‑exchange resin column and refractive index detection.

Q: Who typically uses it?

A: Food and ingredient manufacturers (syrup and starch processors), quality control and analytical laboratories, standards organizations and regulatory analysts who need a reproducible method to quantify sugars and related polyols in syrup matrices.

Q: Is it current or superseded?

A: The 2013 second edition of ISO 10504 is published and was reviewed/confirmed as current in ISO catalogue reviews; it supersedes the earlier 1998 edition. Users should check their national standards body for any national adoption (e.g., EN ISO versions) or later amendments before purchase.

Q: Is it part of a series?

A: ISO 10504 is a standalone analytical method for starch derivatives; it is not part of the ISO 105 textile colour‑fastness series despite the similarity in number. It is often used in conjunction with other analytical standards for syrup testing (for example ISO 5381 and ISO 3696).

Q: What are the key keywords?

A: HPLC, cation‑exchange, glucose syrup, fructose syrup, hydrogenated glucose syrup, starch derivatives, sugar analysis, sorbitol, mannitol, maltitol.