ISO TS 7833-2024 PDF

Full title and description

Nanotechnologies — Extraction method of nanomaterials from lung tissue by proteinase K digestion. This Technical Specification specifies a laboratory method that uses proteinase K digestion to extract and recover nanomaterials deposited in lung tissue, with guidance on applicability, advantages and separation of particulate and ionic forms.

Abstract

This document provides an extraction method using proteinase K (PK) for nanomaterials deposited in lung tissue. It explains the PK digestion procedure, highlights the advantages and limitations of the method, and gives examples of nanomaterials to which the method can be applied. The method focuses on extracting particles from lung tissue and separating nanoparticles from their ionic counterparts and is intended for particles that remain insoluble during PK digestion.

General information

• Status: Published.
• Publication date: January 2024.
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 07.120 (Nanotechnologies).
• Edition / version: Edition 1 (2024-01).
• Number of pages: 14 pages.

Scope

This Technical Specification defines a laboratory extraction procedure based on proteinase K digestion to recover nanomaterials from lung tissue samples. It provides procedural steps, describes the types of nanomaterials and tissue conditions for which the method is appropriate, and addresses separation of particulate versus ionic species. The method is intended for research and testing contexts where insoluble particles are expected to persist through PK digestion and where reliable recovery from biological tissue is required.

Key topics and requirements

• Proteinase K (PK) digestion procedure for lung tissue sample preparation.
• Recommendations for sample handling, homogenization and digestion conditions (enzyme concentration, temperature, incubation time).
• Steps for separating recovered nanoparticles from ionic species and soluble fractions.
• Quality controls and blank/sample controls to validate extraction and minimize contamination.
• Applicability statements describing types of nanomaterials for which the method is suitable (insoluble particles) and known limitations.
• Guidance on documentation, reporting of recovery efficiency and procedural parameters for reproducibility.

Typical use and users

Primary users include nanotoxicology and inhalation toxicology laboratories, academic researchers studying particle deposition and clearance, regulatory laboratories evaluating lung burden from inhalation studies, manufacturers and quality labs working with engineered nanomaterials, and standards bodies or interlaboratory comparison organizers. The specification is intended to support reproducible extraction and characterization workflows used in exposure and hazard assessment.

Related standards

Relevant ISO technical specifications and standards in the nanotechnology and inhalation/toxicology space include other ISO/TS documents addressing lung burden measurement, nanoparticle characterization and test methods for nanomaterials (for example, ISO/TS documents on lung burden mass measurement, nanoparticle characterization for inhalation testing and related vocabulary and measurement guidance). National adoption or PD (published draft/adopted) versions may be available from national bodies.

Keywords

nanotechnologies, proteinase K, lung tissue extraction, nanomaterials, nanoparticles, extraction method, nanotoxicology, sample digestion, particle recovery, separation of ionic species.

FAQ

Q: What is this standard?

A: ISO/TS 7833:2024 is a Technical Specification from ISO (International Organization for Standardization) that specifies an extraction method using proteinase K to recover nanomaterials from lung tissue samples. It was published as Edition 1 in January 2024.

Q: What does it cover?

A: It covers the laboratory procedure for proteinase K digestion of lung tissue, steps for separating nanoparticles from ionic or soluble fractions, applicability to types of nanomaterials (primarily insoluble particles), and guidance on controls and reporting to ensure reproducible recovery and documentation.

Q: Who typically uses it?

A: Users include research and testing laboratories in nanotoxicology and inhalation toxicology, regulatory testing labs, academic researchers studying particle deposition and clearance, and industry labs involved in characterization or safety assessment of engineered nanomaterials.

Q: Is it current or superseded?

A: As published in January 2024, ISO/TS 7833:2024 is a current Technical Specification (Edition 1). Users should check for any subsequent amendments or revisions from ISO or national adoption bodies for later changes.

Q: Is it part of a series?

A: ISO/TS 7833:2024 sits within ISO's broader portfolio of nanotechnologies standards and technical specifications (ISO/TC 229). It complements other ISO/TS documents on nanoparticle measurement, lung burden assessment and nanomaterial characterization rather than being a numbered multi-part series itself.

Q: What are the key keywords?

A: Key keywords are: nanotechnologies, proteinase K, lung tissue, extraction method, nanoparticles, nanomaterials, nanotoxicology, sample digestion, particle recovery, separation.