Overview
EN 18196:2026 - Algae and Algae Products: Determination of Inorganic Arsenic by Anion-Exchange (HPLC-ICP-MS) sets a standardized method for quantifying inorganic arsenic in algae and algae-derived products using anion-exchange High Performance Liquid Chromatography coupled with Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP-MS). This document, developed by CEN, supports laboratories and businesses operating in the seaweed, biobased products, and food safety sectors, ensuring accurate detection and regulatory compliance for inorganic arsenic content.
The method is optimized for seaweeds containing inorganic arsenic and arsenosugar 408 (sulfate-arsinoylriboside) and includes clear separation of these compounds by gradient elution. Validated through interlaboratory studies for Ascophyllum nodosum and Saccharina latissima, the method is also applicable to other algae species, promoting broader adoption across industry and research laboratories.
Key Topics
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Scope and Target Analytes
- Determination of inorganic arsenic (arsenite and arsenate) in algae, seaweed, and algae-derived products
- Specific detection and separation of arsenosugar 408 alongside inorganic arsenic species
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Sample Extraction and Preparation
- Water bath extraction using diluted nitric acid and hydrogen peroxide to oxidize and solubilize arsenic species
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Analytical Procedure
- Use of anion-exchange HPLC for chromatographic separation of arsenic compounds
- ICP-MS used for detection and quantification of inorganic arsenic
- Matrix-matched calibration solutions and quality control practices to ensure traceability and accuracy
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Precision and Method Validation
- Repeatability and reproducibility established through interlaboratory studies, supporting method reliability
- Detailed guidance on avoiding contamination and handling blanks
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Reporting Requirements
- Standardized structure for reporting results, ensuring transparency and comparability
Applications
Related Standards
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EN 17399: Algae and algae products - Vocabulary
Provides terminology necessary for standardization in the algae sector.
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EN 16802: Foodstuffs - Determination of inorganic arsenic by anion-exchange HPLC-ICP-MS
Sets guidelines for quantifying inorganic arsenic in foodstuffs, complementing EN 18196 for edible algae.
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EN 17374: Animal feeding stuffs - Determination of inorganic arsenic by HPLC-ICP-MS
Methods for assessing arsenic in animal feed, relevant for algae-based feed additives.
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EN 17605: Algae and algae products - Methods of sampling and analysis - Sample treatment
Guidance for proper sample collection and preparation, vital for reliable testing.
Practical Value
Adopting EN 18196:2026 enables laboratories and manufacturers to achieve accurate, consistent measurements of inorganic arsenic in algae and algae products. By following a harmonized protocol, organizations can demonstrate due diligence in food safety, support market access, and build consumer trust in algae-derived products. This standard underpins quality control, risk assessment, and regulatory oversight in the growing biobased product sector, emphasizing safety and sustainability.