EN ISO 10705-3:2024 PDF

Water quality - Detection and enumeration of bacteriophages - Part 3: Validation of methods for concentration of bacteriophages from water (ISO 10705-3:2003)

Название (английский):

EN ISO 10705-3:2024

Water quality - Detection and enumeration of bacteriophages - Part 3: Validation of methods for concentration of bacteriophages from water (ISO 10705-3:2003)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 20
Дата публикации:: 14 августа 2024 г.
Издание:: CEN EN 10705 edition 1 version 1
ICS:: 07.100.20

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Описание (английский):

ISO 10705-3:2003 specifies the general principles for assessing the performance of methods for the concentration of bacteriophages from water. Concentration is recommended for those water samples expected to contain < 3 pfp (plaque-forming particles) per millilitre. Concentration methods can be applied to all kinds of water provided that the amount and nature of suspended solids and/or dissolved matter do not interfere with the concentration procedure. ISO 10705-3:2003 does not give specific details of concentration methods, but outlines the fundamental principles for evaluating the suitability of a particular method for a given type and volume of water. Annex A gives examples of methods that have been found satisfactory and their fields of application.

Технические детали

Технический комитет: CEN/TC 230 - Water analysis
SKU: EN ISO 10705-3:2024

Abstract

Overview

EN ISO 10705-3:2024 (identical to ISO 10705-3:2003) defines the principles for validating methods that concentrate bacteriophages from water prior to detection and enumeration. The standard addresses when and how to concentrate large-volume water samples (from 100 ml up to several litres) into smaller analytical volumes (typically a few ml up to 20 ml) so that phage assays (plaque-forming particles, pfp) can be carried out reliably. Concentration is recommended when samples are expected to contain less than 3 pfp per millilitre. Annex A lists example concentration techniques and their suitable fields of application.

Key topics and technical requirements

Scope and applicability: Applies to all kinds of water provided suspended solids or dissolved matter do not interfere with the concentration step.
Validation principle: Evaluate a concentration method by determining phage recovery and reproducibility using samples spiked with naturally polluted water (e.g., primary or treated sewage).
Sampling guidance: Samples up to 10 L are routinely transportable; for larger volumes, initial concentration in the field is recommended. Transport and storage of intermediate concentrates must be included in validation.
Spiking and controls: Prepare sewage spiking material (centrifuge at ~1 000 g for 20 min or filter through 8–12 µm), aim for target seeding concentrations (example ranges 60–200 pfp/ml before aliquoting), add 5% glycerol for storage, freeze at specified temperatures and verify homogeneity.
Analytical quality: Assess recovery across volumes and turbidity levels, monitor reproducibility and within/between-batch variation (statistical tests such as Cochran’s dispersion are referenced).
Safety and equipment: Follow microbiological laboratory safety; some concentration steps may involve pressure - observe relevant precautions.
Non-prescriptive: The standard does not mandate specific concentration techniques but provides the validation framework; Annex A offers representative methods proven satisfactory.

Applications and users

Environmental and water-quality laboratories validating phage concentration workflows for routine monitoring.
Wastewater and drinking-water surveillance teams assessing viral indicators.
Research groups developing or comparing phage concentration methods (filtering, precipitation, adsorption-elution, etc.).
Public health agencies, consultants, and method developers implementing standard-compliant protocols for bacteriophage recovery and enumeration.

Related standards

ISO 10705-1, ISO 10705-2, ISO 10705-4 (other parts of the bacteriophage series)
ISO 8199 (general microbiological enumeration guidance)
ISO 3696 (water for analytical laboratory use)
ISO 6887-1 (sample preparation)
ISO 19458 (sampling for microbiological analysis)

Keywords: EN ISO 10705-3:2024, bacteriophages, water quality, concentration methods, phage concentration validation, plaque-forming particles, water microbiology.

Overview

Key topics and technical requirements

Scope and applicability: Applies to all kinds of water provided suspended solids or dissolved matter do not interfere with the concentration step.
Validation principle: Evaluate a concentration method by determining phage recovery and reproducibility using samples spiked with naturally polluted water (e.g., primary or treated sewage).
Sampling guidance: Samples up to 10 L are routinely transportable; for larger volumes, initial concentration in the field is recommended. Transport and storage of intermediate concentrates must be included in validation.
Spiking and controls: Prepare sewage spiking material (centrifuge at ~1 000 g for 20 min or filter through 8–12 µm), aim for target seeding concentrations (example ranges 60–200 pfp/ml before aliquoting), add 5% glycerol for storage, freeze at specified temperatures and verify homogeneity.
Analytical quality: Assess recovery across volumes and turbidity levels, monitor reproducibility and within/between-batch variation (statistical tests such as Cochran’s dispersion are referenced).
Safety and equipment: Follow microbiological laboratory safety; some concentration steps may involve pressure - observe relevant precautions.
Non-prescriptive: The standard does not mandate specific concentration techniques but provides the validation framework; Annex A offers representative methods proven satisfactory.

Applications and users

Environmental and water-quality laboratories validating phage concentration workflows for routine monitoring.
Wastewater and drinking-water surveillance teams assessing viral indicators.
Research groups developing or comparing phage concentration methods (filtering, precipitation, adsorption-elution, etc.).
Public health agencies, consultants, and method developers implementing standard-compliant protocols for bacteriophage recovery and enumeration.

Related standards

ISO 10705-1, ISO 10705-2, ISO 10705-4 (other parts of the bacteriophage series)
ISO 8199 (general microbiological enumeration guidance)
ISO 3696 (water for analytical laboratory use)
ISO 6887-1 (sample preparation)
ISO 19458 (sampling for microbiological analysis)

Keywords: EN ISO 10705-3:2024, bacteriophages, water quality, concentration methods, phage concentration validation, plaque-forming particles, water microbiology.

EN ISO 10705-3:2024 PDF

Технические детали

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Похожие стандарты

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Похожие стандарты

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