EN ISO 20418-3:2020 PDF

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020)

Название (английский):

EN ISO 20418-3:2020

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 32
Дата публикации:: 1 июля 2020 г.
Издание:: CEN EN 20418 edition 1 version 1
ICS:: 59.060.01

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Описание (английский):

This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction. NOTE 1 The composition of non-animal hair fibres can be measured by ISO 1833 (all parts). Both results are combined to determine the total fibre composition. The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827[4]. It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present. NOTE 2 In this case, the quantitative analysis is performed using microscopical analysis [for example, ISO 17751 (all parts)].

Технические детали

Технический комитет: CEN/TC 248 - Textiles and textile products
SKU: EN ISO 20418-3:2020

Abstract

Overview

EN ISO 20418-3:2020 (ISO 20418-3:2020) specifies a qualitative and quantitative proteomic procedure for determining the composition of animal hair fibre blends using LC‑MS without protein reduction. The method targets common luxury and commodity animal fibres-wool, cashmere, yak, alpaca, camel, and angora-and is designed for robust peptide detection after trypsin digestion of powdered fibres. The standard is intended for textile testing laboratories, quality control and authentication of fibre blends (for example, cashmere authentication), and regulatory or forensic investigations involving fibre composition.

Key topics and technical requirements

Scope: Determination of animal hair fibre blend composition by LC‑MS without a reducing step. Non‑animal fibres should be analysed by ISO 1833 and combined with LC‑MS results for total composition.
Preliminary identification: Light microscopy morphological identification (per ISO/TR 11827) to identify fibre types prior to LC‑MS analysis. The method is not applicable when fibres from the same animal species are blended (e.g., cashmere and mohair).
Sample preparation: Degreasing, mechanical powderization of fibres and trypsin digestion to produce peptides suitable for LC‑MS analysis.
Peptide selection: Use of cysteine‑free marker peptides to avoid the need for protein reduction and simplify sample preparation.
LC‑MS analysis: Chromatographic and mass spectrometric detection of marker peptides; the standard includes example LC‑MS conditions and chromatograms.
Quantification procedures: Calculation of correction factors and blending ratios using marker peptide intensities; detailed formulas and worked examples are provided.
Validation and reporting: Criteria for data validity, interlaboratory study results, and recommended test report contents.

Applications and users

Textile testing and analytical laboratories performing fibre blend analysis, especially for high‑value fibres (cashmere, yak).
Manufacturers and brands verifying material claims and preventing mislabelling or adulteration.
Certification bodies, customs and regulatory agencies enforcing fibre content standards.
Forensic and consumer protection organizations needing reliable species identification in processed textiles.

Related standards

ISO 20418-1 - alternative LC‑MS method with protein reduction/extraction.
ISO 1833 (all parts) - quantitative analysis of non‑animal fibres.
ISO/TR 11827 - microscopic identification of hair fibres.
ISO 17751 (all parts) - microscopical quantitative analysis when same‑species fibres are present.

Keywords: EN ISO 20418-3:2020, LC‑MS, peptide detection, proteomic analysis, textiles, animal hair fibres, cashmere authentication, fibre blend analysis, protein reduction.

Overview

Key topics and technical requirements

Scope: Determination of animal hair fibre blend composition by LC‑MS without a reducing step. Non‑animal fibres should be analysed by ISO 1833 and combined with LC‑MS results for total composition.
Preliminary identification: Light microscopy morphological identification (per ISO/TR 11827) to identify fibre types prior to LC‑MS analysis. The method is not applicable when fibres from the same animal species are blended (e.g., cashmere and mohair).
Sample preparation: Degreasing, mechanical powderization of fibres and trypsin digestion to produce peptides suitable for LC‑MS analysis.
Peptide selection: Use of cysteine‑free marker peptides to avoid the need for protein reduction and simplify sample preparation.
LC‑MS analysis: Chromatographic and mass spectrometric detection of marker peptides; the standard includes example LC‑MS conditions and chromatograms.
Quantification procedures: Calculation of correction factors and blending ratios using marker peptide intensities; detailed formulas and worked examples are provided.
Validation and reporting: Criteria for data validity, interlaboratory study results, and recommended test report contents.

Applications and users

Textile testing and analytical laboratories performing fibre blend analysis, especially for high‑value fibres (cashmere, yak).
Manufacturers and brands verifying material claims and preventing mislabelling or adulteration.
Certification bodies, customs and regulatory agencies enforcing fibre content standards.
Forensic and consumer protection organizations needing reliable species identification in processed textiles.

Related standards

ISO 20418-1 - alternative LC‑MS method with protein reduction/extraction.
ISO 1833 (all parts) - quantitative analysis of non‑animal fibres.
ISO/TR 11827 - microscopic identification of hair fibres.
ISO 17751 (all parts) - microscopical quantitative analysis when same‑species fibres are present.

Keywords: EN ISO 20418-3:2020, LC‑MS, peptide detection, proteomic analysis, textiles, animal hair fibres, cashmere authentication, fibre blend analysis, protein reduction.

EN ISO 20418-3:2020 PDF

Технические детали

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Похожие стандарты

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Похожие стандарты

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