ISO 17244:2025 PDF

Water quality — Determination of the toxicity of water samples on the embryo-larval development of the Japanese oyster (Magallana gigas) and the blue mussel (Mytilus edulis or M. galloprovincialis)

Название (английский):

ISO 17244:2025

Water quality — Determination of the toxicity of water samples on the embryo-larval development of the Japanese oyster (Magallana gigas) and the blue mussel (Mytilus edulis or M. galloprovincialis)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 25
Дата публикации:: 22 июля 2025 г.
Издание:: ISO IS 17244 edition 2 version 1
ICS:: 13.060.70

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Описание (английский):

This document specifies a method for assessing the effects of chemical and aqueous samples on the embryo-larval development of marine bivalves. This method allows the determination of the concentration levels that result in an abnormality in embryo-larval development. This test is suitable for salinity ranges: — between 20 PSU (practical salinity unit) and 40 PSU for mussels, and — between 25 PSU and 35 PSU for oysters. This method in this document applies to: — chemical substances and preparations, — marine and brackish waters, — streams and aqueous effluents (urban, agricultural, industrial effluents, etc.) as long as the salinity is adjusted or dilution is limited so that the aforementioned salinity ranges are respected, — aqueous extracts (pore water, elutriates, eluates and leachates) from sediments and petroleum products, and — samples of contaminated sediment or dredged material (see Annex C).

Технические детали

Технический комитет: ISO/TC 147/SC 5 - Biological methods
SKU: ISO 17244:2025

Abstract

Overview

ISO 17244:2025 - Water quality - specifies a standardized embryo‑larval bioassay to determine the toxicity of chemical and aqueous samples on the early development of marine bivalves: the Japanese oyster (Magallana gigas) and the blue mussel (Mytilus edulis or M. galloprovincialis). The method measures concentration levels that cause developmental abnormalities and is suitable for routine marine toxicity testing across defined salinity ranges (20–40 PSU for mussels; 25–35 PSU for oysters). The 2025 second edition updates the 2015 protocol, adds a sediment direct‑contact protocol (Annex C), adopts the revised oyster nomenclature, and permits extending test duration up to 48 h.

Key Topics

Test organisms and conditions
- Use of mature bivalves for gamete collection; guidance on spawning and fertilization.
- Acceptable salinity and temperature ranges for valid tests.
Sample types covered
- Chemical substances and preparations, marine/brackish waters, effluents, aqueous extracts (pore water, eluates, leachates), and contaminated sediments (Annex C).
Test procedure essentials
- Collection/preparation of samples, selection of concentration ranges, gamete collection, fertilization, incubation, and observation of embryo‑larval development.
Endpoints and metrics
- Developmental abnormalities as the primary endpoint; expression of results using effect concentrations (ECx) and LOEC.
Quality and reporting
- Validity criteria, analytical measurements during tests, and required contents of the test report.
Normative and informative references
- ISO 5667‑16 is referenced for sampling and biotesting guidance; performance data and intercalibration history are provided.

Applications

Environmental monitoring of coastal and estuarine water quality using bivalve bioassays.
Regulatory and compliance testing of industrial, agricultural, and municipal effluents discharged to marine environments.
Risk assessment of chemicals, petroleum products, and sediment/dredged material that may affect early life stages of marine bivalves.
Research and inter‑laboratory studies assessing acute teratogenic effects and deriving toxicity thresholds (EC10/EC25/EC50, LOEC).

Who should use this standard

Environmental testing laboratories and aquaculture hatcheries
Regulatory agencies and environmental consultants
Researchers in marine ecotoxicology and sediment assessment
Organizations performing compliance testing for effluent permits and dredging projects

Related Standards

ISO 5667‑16 (sampling - guidance on biotesting of samples)
Referenced ICES recommendations and ISO definitions (e.g., ECx, LOEC) are used for consistent reporting and interpretation.

Keywords: ISO 17244:2025, water quality, embryo‑larval toxicity test, Magallana gigas, Mytilus edulis, bivalve bioassay, marine toxicity testing, sediment toxicity, effluent testing.

Overview

Key Topics

Test organisms and conditions
- Use of mature bivalves for gamete collection; guidance on spawning and fertilization.
- Acceptable salinity and temperature ranges for valid tests.
Sample types covered
- Chemical substances and preparations, marine/brackish waters, effluents, aqueous extracts (pore water, eluates, leachates), and contaminated sediments (Annex C).
Test procedure essentials
- Collection/preparation of samples, selection of concentration ranges, gamete collection, fertilization, incubation, and observation of embryo‑larval development.
Endpoints and metrics
- Developmental abnormalities as the primary endpoint; expression of results using effect concentrations (ECx) and LOEC.
Quality and reporting
- Validity criteria, analytical measurements during tests, and required contents of the test report.
Normative and informative references
- ISO 5667‑16 is referenced for sampling and biotesting guidance; performance data and intercalibration history are provided.

Applications

Environmental monitoring of coastal and estuarine water quality using bivalve bioassays.
Regulatory and compliance testing of industrial, agricultural, and municipal effluents discharged to marine environments.
Risk assessment of chemicals, petroleum products, and sediment/dredged material that may affect early life stages of marine bivalves.
Research and inter‑laboratory studies assessing acute teratogenic effects and deriving toxicity thresholds (EC10/EC25/EC50, LOEC).

Who should use this standard

Environmental testing laboratories and aquaculture hatcheries
Regulatory agencies and environmental consultants
Researchers in marine ecotoxicology and sediment assessment
Organizations performing compliance testing for effluent permits and dredging projects

Related Standards

ISO 5667‑16 (sampling - guidance on biotesting of samples)
Referenced ICES recommendations and ISO definitions (e.g., ECx, LOEC) are used for consistent reporting and interpretation.

Keywords: ISO 17244:2025, water quality, embryo‑larval toxicity test, Magallana gigas, Mytilus edulis, bivalve bioassay, marine toxicity testing, sediment toxicity, effluent testing.

ISO 17244:2025 PDF

Технические детали

Abstract

Overview

Key Topics

Applications

Who should use this standard

Related Standards

Похожие стандарты

Abstract

Overview

Key Topics

Applications

Who should use this standard

Related Standards

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