Overview
ISO 18862:2025 - "Coffee and coffee products - Determination of acrylamide" - specifies validated laboratory methods to measure acrylamide in coffee and coffee-derived products. The standard covers sample extraction with water, clean‑up by solid‑phase extraction (SPE) (and optional Carrez precipitation), and quantification by HPLC‑MS/MS or by GC‑MS after derivatization (bromination). Methods were validated across matrices including roasted coffee, soluble coffee, coffee substitutes and coffee products with a validated concentration range from 53 μg/kg to 612.1 μg/kg.
Key technical topics and requirements
- Extraction & clean‑up
- Water extraction or dissolution for soluble products.
- Matrix clean‑up using SPE; Carrez precipitation is included as an option to remove interferents.
- Analytical methods
- HPLC‑MS/MS: direct measurement using tandem mass spectrometry.
- GC‑MS after derivatization: requires bromination of acrylamide prior to GC‑MS analysis.
- Standards and calibration
- Use of isotopically labelled internal standard (D3‑acrylamide; C‑13 labelled standard is allowed as an alternative).
- Guidance on calibration, linearity, working range, laboratory recovery and measurement uncertainty.
- Reagents and safety
- Specifies reagent grades (MS‑grade solvents, water per ISO 3696) and warns about acrylamide hazards - use fume hoods and protective measures.
- Validation and performance
- Precision data (repeatability, reproducibility), recovery, and uncertainty guidance are provided.
- Informative annexes include performance characteristics (Annex A), absorber materials (Annex B), example columns/conditions (Annex C) and HPLC‑MS/MS sample prep examples (Annex D).
- Documentation
- Requirements for test reports and identification criteria for peak confirmation.
Practical applications and users
Who uses ISO 18862:2025:
- Food testing laboratories performing routine acrylamide monitoring in coffee.
- Coffee manufacturers and quality‑control teams validating product safety and process changes.
- Regulatory agencies and compliance bodies setting or enforcing acrylamide limits.
- Research labs studying acrylamide formation, mitigation strategies or processing effects.
Typical uses:
- Regulatory compliance testing and reporting.
- Internal quality control and supplier verification.
- Method transfer, proficiency testing and inter‑laboratory comparisons.
- Research into roasting/processing impacts on acrylamide levels.
Related standards
- ISO 3696 - Water for analytical laboratory use (referenced for reagent water quality).
- The standard is the second edition and replaces ISO 18862:2016; consult national bodies for implementation and patent notices.
Keywords: ISO 18862:2025, acrylamide in coffee, HPLC‑MS/MS, GC‑MS derivatization, solid‑phase extraction (SPE), coffee product testing, acrylamide validation.