ISO 22160:2007 PDF

Milk and milk-based drinks — Determination of alkaline phosphatase activity — Enzymatic photo-activated system (EPAS) method

Open preview (PDF)

Title (English):

ISO 22160:2007

Milk and milk-based drinks — Determination of alkaline phosphatase activity — Enzymatic photo-activated system (EPAS) method

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 16
Publication date:: March 28, 2007
Edition:: ISO IS 22160 edition 1 version 1
ICS:: 67.100.10

Delivery type:

Full version25 €Read online 24h10 €Read online 72h15 €

Document language:

enru

Delivery time:Immediate download

Price:25 €

Description (English):

ISO 22160|IDF 209:2007 specifies a method for the determination of the alkaline phosphatase activity in pasteurized whole milk, semi-skimmed milk, skimmed milk, cream and flavoured milks using a chemiluminescent (EPAS) method. The method is applicable to milk and milk-based drinks from cows, sheep, buffalo and goats. The method is also suitable for any liquid-based sample if diluted in such a way that the diluted alkaline phosphatase activity has less than 7 000 milliunits per litre.

Technical details

Technical committee: ISO/TC 34/SC 5 - Milk and milk products
SKU: ISO 22160:2007

Abstract

Overview

ISO 22160:2007 (IDF 209:2007) specifies an Enzymatic Photo-Activated System (EPAS) chemiluminescent method for determining alkaline phosphatase (ALP) activity in milk and milk‑based drinks. The method is validated for pasteurized whole, semi‑skimmed, skimmed milks, cream and flavoured milks from cows, sheep, buffalo and goats, and is suitable for any liquid sample diluted so the ALP activity is below 7 000 mU/L. Results are expressed in milliunits per litre (mU/L).

Key topics and technical requirements

Principle: ALP hydrolyses a stable aromatic dioxetane‑phosphate substrate at 35 °C ± 1 °C to produce a photo‑activated chemiluminescent product. Signal amplification uses an enhancing macromolecular component and is measured by a luminometer.
Reagents: non‑chemiluminescent dioxetane ester substrate, stopping solution (0.15 mol/L 2‑amino‑2‑methyl‑1‑propanol with 0.02% benzalkonium chloride, pH 10.7), calibrator tablets and positive control enzyme.
Calibration & controls: calibration tablets (fluid or cream/flavoured calibrators) create standard curves (typical calibrator activities noted), and a freeze‑dried positive control (e.g., 450 mU/L after rehydration) is used for QA.
Apparatus: luminometer (operating ~540 nm, with software converting luminescence to enzyme units), mini‑vials, pipettes, incubator/dry block (35 °C ± 1 °C and higher temp baths for controls), water bath, balance, test tubes and printer.
Sample preparation: mix and test samples at refrigeration temperature (0–7 °C); raw milk requires dilution with ALP‑free milk so diluted activity

Related standards

Other ISO standards

Overview

Key topics and technical requirements

Principle: ALP hydrolyses a stable aromatic dioxetane‑phosphate substrate at 35 °C ± 1 °C to produce a photo‑activated chemiluminescent product. Signal amplification uses an enhancing macromolecular component and is measured by a luminometer.
Reagents: non‑chemiluminescent dioxetane ester substrate, stopping solution (0.15 mol/L 2‑amino‑2‑methyl‑1‑propanol with 0.02% benzalkonium chloride, pH 10.7), calibrator tablets and positive control enzyme.
Calibration & controls: calibration tablets (fluid or cream/flavoured calibrators) create standard curves (typical calibrator activities noted), and a freeze‑dried positive control (e.g., 450 mU/L after rehydration) is used for QA.
Apparatus: luminometer (operating ~540 nm, with software converting luminescence to enzyme units), mini‑vials, pipettes, incubator/dry block (35 °C ± 1 °C and higher temp baths for controls), water bath, balance, test tubes and printer.
Sample preparation: mix and test samples at refrigeration temperature (0–7 °C); raw milk requires dilution with ALP‑free milk so diluted activity

ISO 22160:2007 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Related standards

Abstract

Overview

Key topics and technical requirements

Related standards

Information technology — Information security incident management — Part 2: Guidelines to plan and prepare fo…

Risk management — Guidelines

Ships and marine technology — Specification for bunkering of liquefied natural gas fuelled vessels

Information technology — Information security incident management — Part 1: Principles and process

Information technology — Artificial intelligence (AI) — AI system impact assessment

Information technology — Information security incident management — Part 3: Guidelines for ICT incident respo…

Innovation management system — Requirements

Ships and marine technology — Offshore wind energy — Port and marine operations