ISO 22160:2007 PDF

Milk and milk-based drinks — Determination of alkaline phosphatase activity — Enzymatic photo-activated system (EPAS) method

Название (английский):

ISO 22160:2007

Milk and milk-based drinks — Determination of alkaline phosphatase activity — Enzymatic photo-activated system (EPAS) method

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 16
Дата публикации:: 28 марта 2007 г.
Издание:: ISO IS 22160 edition 1 version 1
ICS:: 67.100.10

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Описание (английский):

ISO 22160|IDF 209:2007 specifies a method for the determination of the alkaline phosphatase activity in pasteurized whole milk, semi-skimmed milk, skimmed milk, cream and flavoured milks using a chemiluminescent (EPAS) method. The method is applicable to milk and milk-based drinks from cows, sheep, buffalo and goats. The method is also suitable for any liquid-based sample if diluted in such a way that the diluted alkaline phosphatase activity has less than 7 000 milliunits per litre.

Технические детали

Технический комитет: ISO/TC 34/SC 5 - Milk and milk products
SKU: ISO 22160:2007

Abstract

Overview

ISO 22160:2007 (IDF 209:2007) specifies an Enzymatic Photo-Activated System (EPAS) chemiluminescent method for determining alkaline phosphatase (ALP) activity in milk and milk‑based drinks. The method is validated for pasteurized whole, semi‑skimmed, skimmed milks, cream and flavoured milks from cows, sheep, buffalo and goats, and is suitable for any liquid sample diluted so the ALP activity is below 7 000 mU/L. Results are expressed in milliunits per litre (mU/L).

Key topics and technical requirements

Principle: ALP hydrolyses a stable aromatic dioxetane‑phosphate substrate at 35 °C ± 1 °C to produce a photo‑activated chemiluminescent product. Signal amplification uses an enhancing macromolecular component and is measured by a luminometer.
Reagents: non‑chemiluminescent dioxetane ester substrate, stopping solution (0.15 mol/L 2‑amino‑2‑methyl‑1‑propanol with 0.02% benzalkonium chloride, pH 10.7), calibrator tablets and positive control enzyme.
Calibration & controls: calibration tablets (fluid or cream/flavoured calibrators) create standard curves (typical calibrator activities noted), and a freeze‑dried positive control (e.g., 450 mU/L after rehydration) is used for QA.
Apparatus: luminometer (operating ~540 nm, with software converting luminescence to enzyme units), mini‑vials, pipettes, incubator/dry block (35 °C ± 1 °C and higher temp baths for controls), water bath, balance, test tubes and printer.
Sample preparation: mix and test samples at refrigeration temperature (0–7 °C); raw milk requires dilution with ALP‑free milk so diluted activity

Overview

Key topics and technical requirements

Principle: ALP hydrolyses a stable aromatic dioxetane‑phosphate substrate at 35 °C ± 1 °C to produce a photo‑activated chemiluminescent product. Signal amplification uses an enhancing macromolecular component and is measured by a luminometer.
Reagents: non‑chemiluminescent dioxetane ester substrate, stopping solution (0.15 mol/L 2‑amino‑2‑methyl‑1‑propanol with 0.02% benzalkonium chloride, pH 10.7), calibrator tablets and positive control enzyme.
Calibration & controls: calibration tablets (fluid or cream/flavoured calibrators) create standard curves (typical calibrator activities noted), and a freeze‑dried positive control (e.g., 450 mU/L after rehydration) is used for QA.
Apparatus: luminometer (operating ~540 nm, with software converting luminescence to enzyme units), mini‑vials, pipettes, incubator/dry block (35 °C ± 1 °C and higher temp baths for controls), water bath, balance, test tubes and printer.
Sample preparation: mix and test samples at refrigeration temperature (0–7 °C); raw milk requires dilution with ALP‑free milk so diluted activity

ISO 22160:2007 PDF

Технические детали

Abstract

Overview

Key topics and technical requirements

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Abstract

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