Foodstuffs. DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments
Foodstuffs. DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments
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What is PD CEN/TS 17303 – DNA barcoding for fish about?
PD CEN/TS 17303 describes a procedure for the identification of single fish and fish fillets to the level of genus or species. The identification of fish species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI) [2], [3] or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases. The methodology allows the identification of a large number of commercially important fish species. PD CEN/TS 17303 is usually unsuitable for the analysis of highly processed foods, e.g., tins of fish, with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets. Furthermore, it is not applicable for complex fish products containing mixtures of two or more fish species.
Who is PD CEN/TS 17303 – DNA barcoding for fish for
Другие стандарты PD