Foodstuffs. General guidelines for the validation of qualitative real-time PCR methods - Single-laboratory validation
Foodstuffs. General guidelines for the validation of qualitative real-time PCR methods - Single-laboratory validation
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What is this standard about? This document describes the performance characteristics and minimum performance criteria which should be taken into account when conducting a single-laboratory validation study for qualitative (binary) real-time polymerase chain reaction (PCR) methods applied for the detection of specific DNA sequences present in foods. The protocol was developed for qualitative real-time PCR methods for the detection of DNA sequences derived from genetically modified foodstuffs. It is applicable also for single-laboratory validation of qualitative PCR methods used for analysis of other food materials, e.g. for species detection and identification.
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